9 - Overlord
14 - Spawning Pool
15 - Hatchery
First Queen @ Spawning Pool
17 - Overlord
18 - 4 Zerglings
Second Queen @ First Queen (Send to Natural)
24 - Overlord
Third Queen @ Natural
Fourth Queen @ Second Queen
32 - Double Gas x3 @ 5min
32 - Overlord

@100 Gas: Lair & Evolution Chamber