Values;Date.Col
911;20/12/2013 04:05:01 p.m.
124,82;23/12/2013 09:43:03 a.m.
287;23/12/2013 09:44:15 a.m.
37,3;23/12/2013 09:45:26 a.m.
448,4;23/12/2013 09:46:50 a.m.
432,5;23/12/2013 09:48:31 a.m.

df <- read.table("tmp2.csv", header = T, dec = ',', sep = ';')
df$Date.Col <- strptime(df$Date.Col, "%d/%m/%Y %I:%M:%S %p")
str(df)
plot(Values ~ Date.Col, df, type = 'l')

Error in (function (formula, data = NULL, subset = NULL, na.action = na.fail,  :
  invalid type (list) for variable 'Date.Col'