row_to_add = pd.MultiIndex.from_tuples()
df.ix[row_to_add] = my_row

>>> df = pd.DataFrame({'Time': [dt.datetime(2013,2,3,9,0,1), dt.datetime(2013,2,3,9,0,1)], 'hsec': [1,25], 'vals': [45,46]})
>>> df
                 Time  hsec  vals
0 2013-02-03 09:00:01     1    45
1 2013-02-03 09:00:01    25    46

[2 rows x 3 columns]
>>> df.set_index(['Time','hsec'],inplace=True)
>>> ind = pd.MultiIndex.from_tuples([(dt.datetime(2013,2,3,9,0,2),0)],names=['Time','hsec'])
>>> df.ix[ind] = 5

Traceback (most recent call last):
  File "<pyshell#201>", line 1, in <module>
    df.ix[ind] = 5
  File "C:Program FilesPython27libsite-packagespandascoreindexing.py", line 96, in __setitem__
    indexer = self._convert_to_indexer(key, is_setter=True)
  File "C:Program FilesPython27libsite-packagespandascoreindexing.py", line 967, in _convert_to_indexer
    raise KeyError('%s not in index' % objarr[mask])
KeyError: "[(Timestamp('2013-02-03 09:00:02', tz=None), 0L)] not in index"