TemplateField tf_ForMalls = new TemplateField();
    tf_ForMalls.HeaderText = "For Malls";

    CheckBox chk_ForMalls = new CheckBox();
    chk_ForMalls.ID = "chkDelete";

    tf_ForMalls.ItemTemplate = chk_ForMalls as ITemplate;

    dv_SpotDetail.Fields.Add(tf_ForMalls);

public class MyTemplate : ITemplate
{
    #region ITemplate Members

    public void InstantiateIn(Control container)
    {
        CheckBox chk = new CheckBox();
        chk.ID = "chk";
        container.Controls.Add(chk);
    }

    #endregion
}

TemplateField tf = new TemplateField();
tf.ItemTemplate = new MyTemplate();
detvw.Fields.Add(tf);

CheckBox chk_Eliminar = (CheckBox)item.FindControl("chk");