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Can Mono DataConvert be used to read a 3-byte integer?
public Region(string regionFile)
{
    var conv = DataConverter.BigEndian;
    var chunks = new Chunk[Chunk.MaxLocations];
    using (var fs = File.OpenRead(regionFile))
    {
        var buffer = new byte[4096];
        fs.Read(buffer, 0, buffer.Length);

        // Read chunk locations
        for (int i = 0; i < Chunk.MaxLocations; ++i)
        {
            chunks[i] = new Chunk(conv.ToInt24(buffer, i * 4), buffer[i * 4 + 3]);
        }
    }
}

public override int GetInt24(byte[] data, int index)
{
    if (data == null)
        throw new ArgumentNullException("data");
    if (data.Length - index < 3)
        throw new ArgumentException("index");
    if (index < 0)
        throw new ArgumentException("index");

    int ret = 0;
    byte* b = (byte*)&ret;

    for (int i = 0; i < 3; i++)
        b[2 - i] = data[index + i];

    return ret;
}