self.firstRadioButton = Radiobutton(self.__canvas, text="ONE", fg='white', bg=BACKGROUND_COLOR, variable=self.selectedONE, value=1)

self.secondRadioButton = Radiobutton(self.__canvas, text="TWO", fg='white', bg=BACKGROUND_COLOR, variable=self.selectedTWO, value=2)

import tkinter


# function that is called when you select a certain radio button
def selected():
    print(var.get())


root = tkinter.Tk()


var = tkinter.StringVar() #used to get the 'value' property of a tkinter.Radiobutton

# Note that I added a command to each radio button and a different 'value'
# When you press a radio button, its corresponding 'command' is called.
# In this case, I am linking both radio buttons to the same command: 'selected'

rb1 = tkinter.Radiobutton(text='Radio Button 1', variable=var, value="Radio 1", command=selected)
rb1.pack()
rb2 = tkinter.Radiobutton(text='Radio Button 2', variable=var, value="Radio 2", command=selected)
rb2.pack()


root.mainloop()

self.secondRadioButton.bind('<ButtonRelease-1>', self.__function_name_that_should_run_when_radiobtn_selected)

#!/usr/bin/env python

from Tkinter import Tk, Frame, Label, Entry, Button, TOP, LEFT, RIGHT, X, YES, END,
    Radiobutton, StringVar,
    Canvas


def padded(text):
    # return a padded 80-column string
    line = text
    line += (30 - len(text)) * ' '
    return line


class PRB:
    def __init__(self, master):
        # self.entries = {}
        frame = Frame(master)
        frame.pack(side=TOP, fill=X, expand=YES, padx=5, pady=5)
        root.geometry("360x220")

        self.w = Canvas(master, width=560, height=400)
        self.w.pack()
        self.w.create_line(11, 5, 330, 5)
        self.w.create_line(11, 37, 330, 37)
        self.w.create_line(11, 73, 330, 73)
        self.w.create_line(11, 5, 11, 73)
        self.w.create_line(110, 5, 110, 73)
        self.w.create_line(330, 5, 330, 73)
        self.w.create_line(275, 5, 275, 73)

        self.ent1 = Entry(master)
        self.ent1.pack(side=RIGHT, expand=YES, fill=X)
        self.ent1.place(x=130, y=100, width=130)
        self.ent1.delete(0, END)
        self.ent1.insert(0, 'Enter a pass to pass!')
        self.ent1.focus_set()

        self.ent2 = Entry(master)
        self.ent2.pack(side=RIGHT, expand=YES, fill=X)
        self.ent2.place(x=130, y=140, width=130)
        self.ent2.delete(0, END)
        self.ent2.insert(0, 'Enter a pass to pass!')
        self.ent2.focus_set()

        self.v = StringVar()
        self.rb1 = Radiobutton(root, text="Type 1", variable=self.v, value=1)
        self.rb1.pack(side=TOP)
        self.rb1.place(x=20, y=20)

        self.rb2 = Radiobutton(root, text="Type 2", variable=self.v, value=2)
        self.rb2.pack(side=TOP)
        self.rb2.place(x=20, y=55)

        self.b1 = Button(master, width=10, text='Test_X', command=self.Test_X)
        self.b1.pack(side=RIGHT)
        self.b1.place(x=20, y=100)

        self.b2 = Button(master, width=10, text='Test_Y', command=self.Test_Y)
        self.b2.pack(side=RIGHT)
        self.b2.place(x=20, y=140)

        self.quit = Button(master, width=10, text='Cancel', bg='red', fg='white', command=root.quit)
        self.quit.pack(side=LEFT)
        self.quit.place(x=20, y=180)

        self.pasMSG = 'PASS'
        self.pasMSG2 = 'PASS'

        self.msg = padded('Please, select a type!')
        self.l1 = Label(root, text=self.msg, anchor='w')
        self.l1.pack()
        self.l1.place(x=120, y=20)

        self.l2 = Label(master, text=self.msg, anchor='w')
        self.l2.pack(side=RIGHT)
        self.l2.place(x=120, y=55)

        self.p1 = Label(master, text=self.pasMSG, anchor='w')
        self.p1.pack(side=RIGHT)
        self.p1.place(x=285, y=20)

        self.p2 = Label(master, text=self.pasMSG2, anchor='w')
        self.p2.pack(side=RIGHT)
        self.p2.place(x=285, y=55)


    def Test_X(self):
        if self.v.get() == '1':
            msg = padded('Type 1 Test_X')
            self.l1 = Label(root, text=msg, anchor='w')
            self.l1.pack()
            self.l1.place(x=120, y=20)

            if self.ent1.get().strip() == 'pass':
                pasMSG = padded('PASS!')
                co = 'green'
            else:
                pasMSG = padded('FAIL :(')
                co = 'red'
            self.p1 = Label(root, text=pasMSG, bg=co, fg='white', anchor='w')
            self.p1.pack(side=RIGHT)
            self.p1.place(x=285, y=20, width=40)
            # self.p1.config(height = )

        elif self.v.get() == '2':
            msg = padded('Type 2 Test_X')
            self.l1 = Label(root, text=msg, anchor='w')
            self.l1.pack()
            self.l1.place(x=120, y=20)

            if self.ent1.get().strip() == 'pass':
                pasMSG = padded('PASS!')
                co = 'green'
            else:
                pasMSG = padded('FAIL :(')
                co = 'red'
            self.p1 = Label(root, text=pasMSG, bg=co, fg='white', anchor='w')
            self.p1.pack(side=RIGHT)
            self.p1.place(x=285, y=20, width=40)

    def Test_Y(self):
        if self.v.get() == '1':
            msg2 = padded('Type 1 Test_Y')
            self.l2 = Label(root, text=msg2, anchor='w')
            self.l2.pack()
            self.l2.place(x=120, y=55)

            if self.ent2.get().strip() == 'pass':
                pasMSG2 = padded('PASS')
                co = 'green'
            else:
                pasMSG2 = padded('FAIL :(')
                co = 'red'
            self.p2 = Label(root, text=pasMSG2, bg=co, fg='white', anchor='w')
            self.p2.pack(side=RIGHT)
            self.p2.place(x=285, y=55, width=40)
            # self.p1.config(height = )

        elif self.v.get() == '2':
            msg2 = padded('Type 2 Test_Y')
            self.l2 = Label(root, text=msg2, anchor='w')
            self.l2.pack()
            self.l2.place(x=120, y=55)

            if self.ent2.get().strip() == 'pass':
                pasMSG2 = padded('PASS!')
                co = 'green'
            else:
                pasMSG2 = padded('FAIL :(')
                co = 'red'
            self.p2 = Label(root, text=pasMSG2, bg=co, fg='white', anchor='w')
            self.p2.pack(side=RIGHT)
            self.p2.place(x=285, y=55, width=40)


if __name__ == '__main__':
    root = Tk()
    prb = PRB(root)
    root.title('put "pass" in the entries to pass the test!')
    root.mainloop()