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ISOGEN_DERWTEMP_LOGENT(173).txt

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  1. <PNO> WO2008142034A2
  2. <FMT> 2
  3. <DW> 200848
  4. <AEC> 7006
  5. <LA> ENG
  6. <PP> 442
  7. <DWG> 0/6
  8. <TI1> Producing a transgenic plant cell, plant, or its part with increased tolerance and/or resistance to environmental stress and increased biomass production by increasing or generating activities selected from Stress-Related Proteins
  9. <NOV> NOVELTY - Producing a transgenic plant cell, plant, or its part with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, a plant, or its part comprises increasing or generating one or more activities selected from Stress-Related Proteins (SRP), e.g. 2,3-dihydroxy-2,3-dihydrophenylpropionatedehydrogenase, 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, or 3-deoxy-D-arabino-heptu losonate-7-phosphatesynthase.
  10. <DDSC> DETAILED DESCRIPTION - INDEPENDENT CLAIMS are:
  11. (1) a transgenic plant cell, plant, or its part with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part produced by the method above;
  12. (2) a seed produced by the transgenic plant, where the seed is genetically homozygous for a transgene conferring increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part resulting in an increased tolerance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant;
  13. (3) an isolated nucleic acid molecule given in the specification;
  14. (4) a nucleic acid construct which confers the expression of the nucleic acid molecule comprising one or more regulatory elements, where expression of the nucleic acid in a host cell results in increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part;
  15. (5) a vector comprising the nucleic acid molecule or the nucleic acid construct, where expression of the coding nucleic acid in a host cell results in increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part;
  16. (6) a host cell, which has been transformed stably or transiently with the vector, nucleic acid molecule, or nucleic acid construct, and which shows due to the transformation an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part;
  17. (7) a process for producing a polypeptide, where the polypeptide is expressed in the host cell;
  18. (8) a polypeptide produced by the process above or encoded by the nucleic acid molecule, where the polypeptide distinguishes over the sequence given in the specification (Table II) by one or more amino acids;
  19. (9) an antibody, which binds specifically to the polypeptide;
  20. (10) a plant tissue, propagation material, harvested material, or plant comprising the host cell;
  21. (11) a process for the identification of a compound conferring an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part in a plant cell, plant, or its part;
  22. (12) a method for the production of an agricultural composition;
  23. (13) a composition comprising the nucleic acid molecule, polypeptide, nucleic acid construct, vector, compound, antibody, and optionally an agricultural carrier;
  24. (14) an isolated polypeptide given in the specification (Table II, preferably Table II B), which is selected from yeast, preferably <SN>Saccharomyces cerevisiae</SN>, or <SN>Escherichia coli</SN>, preferably <SN>E. coli</SN> K12, and/or <SN>Synechocystis</SN> sp. P00 6803; and
  25. (15) a transgenic plant cell comprising a nucleic acid molecule encoding a polypeptide having an activity selected from SRP, where the polypeptide confers increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part, preferably when the polypeptide is overexpressed.
  26. <TF> BIOTECHNOLOGY - Preferred Method: In the method, the activities are selected from 2,3-dihydroxy-2,3-dihydrophenylpropionatedehydrogenase, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, 3-deoxy-D-arabino-heptu losonate-7-phosphatesynthase, 3-oxoacyl-(acyl carrier protein) synthase, acid shock protein precursor, aspartate ammonia-lyase, b0081-protein, b0482-protein, b0631-protein, b0753-protein, b0866-protein, b1052-protein, b1161-protein, b1423-protein, b1878-protein, b2226-protein, b2475-protein, cellobiose/arbutin/salicin-specific PTS enzyme (IIB component/IC component), Checkpoint protein, CP4-57 prophage/RNase LS, Dihydrouridine synthase, DNA-binding transcriptional dual regulator protein, D-xylose transporter subunit, gamma-Glu-putrescine synthase, gluconate transporter, glucose-1-phosphate thymidylyltransferase, Glutamine tRNA synthetase, glutathione-dependent oxidoreductase, glycine betaine transporter subunit protein, glycogen synthase, GTP cyclohydrolase I, heat shock protein, heat shock
  27. protein HtpX, heme lyase (CcmH subunit), hexuronate transporter, histidine/lysine/arginine/ornithine transporter subunit protein, HyaA/HyaB-processing protein, inner membrane protein, L-arabinose transporter subunit, Lsm (Like Sm) protein, L-threonine 3-dehydrogenase, methylglyoxal synthase, multidrug efflux system (subunit B), N,N'-diacetylchitobiose-specific enzyme IIA component of PTS, NADH dehydrogenase (subunit N), neutral amino-acid efflux system, nicotinamide nucleotide adenylyltransferase, ornithine decarboxylase, pantothenate kinase, peptidyl-prolyl cis-trans isomerase A (rotamase A), phosphate transporter, phosphatidylglycerophosphate synthetase, polyphosphate kinase, potassium-transporting ATPase (subunit B), predicted antimicrobial peptide transporter subunit, predicted arginine/ornithine transporter, predicted hydrolase, predicted kinase, predicted ligase, predicted outer membrane lipoprotein, predicted oxidoreductase (flavin:NADH component), predicted porin, predicted PTS enzymes (IIB component/
  28. IIC component), predicted serine transporter protein, predicted transporter protein, Protein component of the small (40S) ribosomal subunit, regulator of length of O-antigen component of lipopolysaccharide chains, ribonuclease activity regulator protein RraA, sensory histidine kinase in two-component regulatory system with NarP (NarL), sodium/proton antiporter, Splicing factor, threonine and homoserine efflux system, transcriptional regulator protein, transcriptional repressor protein MetJ, transporter subunit/periplasmic-binding component of ABC superfamily, tRNA pseudouridine synthase, tRNA-specific adenosine deaminase, universal stress protein UP12, Yal049c-protein, YCR059C-protein, YEL005C-protein, YER1560-protein, Yfr042w-protein, YGL045W-protein, or YOR024w-protein. The activity of at least one polypeptide selected from: (a) a polypeptide comprising a polypeptide, a consensus sequence, or at least one polypeptide motif given in the specification (Column 5 or 7 of Table II or of Table IV, respectively);
  29. (b) an expression product of a nucleic acid molecule comprising a polynucleotide given in the specification (Column 5 or 7 of Table I); or (c) a functional equivalent of (a) or (b), is increased or generated.
  30. Identifying a compound conferring an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part in a plant cell, plant, or its part, comprises: (a) culturing a plant cell, plant, or its part; maintaining a plant expressing the polypeptide encoded by the nucleic acid molecule conferring an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part; a non-transformed wild type plant or its part and a readout system capable of interacting with the polypeptide under conditions which permit the interaction of the polypeptide with the readout system in the presence of a compound or a sample comprising compounds and capable of providing a detectable signal in response to the binding of a compound to the polypeptide under conditions which permit the expression of the
  31. readout system and of the polypeptide encoded by the nucleic acid molecule; conferring an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part; and (b) identifying if the compound is an agonist by detecting the presence or absence or increase of a signal produced by the readout system. The environmental stress is selected from salinity, drought, temperature, metal, chemical, pathogenic, and/or oxidative stresses. Preferably, the environmental stress is drought and/or desiccation.
  32. Producing an agricultural composition comprises the method above, and formulating the identified compound in a form acceptable for an application in agriculture.
  33. Producing a transgenic plant cell, plant, or its part with increased tolerance and/or resistance to environmental stress and increased biomass production compared to a corresponding non-transformed wild type plant cell, plant, or its part, where the tolerance and/or resistance to environmental stress and increased biomass production is increased by expression of a polypeptide encoded by the nucleic acid and results in increased tolerance and/or resistance to an environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part, comprises: (a) transforming a plant cell, or a part of a plant with the expression vector; and (b) generating from the plant cell or the part of a plant a transgenic plant with an increased tolerance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant.
  34. Producing a transgenic plant with increased biomass production compared to a corresponding non-transformed wild type plant under conditions of environmental stress by increasing or generating one or more activities selected from SRP above. The method comprises: (a) transforming a plant cell or a part of a plant with the expression vector; and (b) generating from the plant cell or the part of a plant a transgenic plant with an increased tolerance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant.
  35. Preferred Nucleic Acid Molecule: The nucleic acid molecule is selected from: (a) a nucleic acid molecule encoding the polypeptide given in the specification (Column 5 or 7 of Table II); (b) a nucleic acid molecule given in the specification (Column 5 or 7 of Table I); (c) a nucleic acid molecule, which, as a result of the degeneracy of the genetic code, can be derived from a polypeptide sequence given in the specification (Column 5 or 7 of Table II) and confers an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, a plant, or its part; (d) a nucleic acid molecule having at least 30% identity with the nucleic acid molecule sequence of a polynucleotide comprising the nucleic acid molecule given in the specification (Column 5 or 7 of Table I) and confers an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed
  36. wild type plant cell, a plant, or its part; (e) a nucleic acid molecule encoding a polypeptide having at least 30% identity with the amino acid sequence of the polypeptide encoded by the nucleic acid molecule of (a) to (c) and having the activity represented by a nucleic acid molecule comprising a polynucleotide given in the specification (Column 5 of Table I) and confers an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, a plant, or its part; (f) nucleic acid molecule which hybridizes with a nucleic acid molecule of (a) to (c) under stringent hybridization conditions and confers an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, a plant, or its part; (g) a nucleic acid molecule encoding a polypeptide which can be isolated with the aid of monoclonal or polyclonal antibodies made
  37. against a polypeptide encoded by one of the nucleic acid molecules of (a) to (e) and having the activity represented by the nucleic acid molecule comprising a polynucleotide given in the specification (Column 5 of Table I); (h) a nucleic acid molecule encoding a polypeptide comprising the consensus sequence or one or more polypeptide motifs given in the specification (Column 7 of Table IV) and preferably having the activity represented by a nucleic acid molecule comprising a polynucleotide given in the specification (Column 5 of Table II or IV); (i) a nucleic acid molecule encoding a polypeptide having the activity represented by a protein given in the specification (Column 5 of Table II) and confers an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, a plant, or its part; (j) nucleic acid molecule which comprises a polynucleotide, which is obtained by amplifying a cDNA library or a genomic
  38. library using the primers given in the specification (Column 7 of Table III) which do not start at their 5'-end with the nucleotides ATA and preferably having the activity represented by a nucleic acid molecule comprising a polynucleotide given in the specification (Column 5 of Table II or IV); or (k) a nucleic acid molecule which is obtainable by screening a nucleic acid library under stringent hybridization conditions with a probe comprising a complementary sequence of a nucleic acid molecule of (a) or (b) or its fragment, having at least 15-500 nt of a nucleic acid molecule complementary to a nucleic acid molecule sequence in (a) to (e) and encoding a polypeptide having the activity represented by a protein comprising a polypeptide given in the specification (Column 5 of Table II), where the expression of the nucleic acid molecule is increased or generated.
  39. Preferred Transgenic Plant Cell, Plant, or Part: The transgenic plant cell, plant, or part is derived from a monocotyledonous plant or a dicotyledonous plant. The plant is selected from maize, wheat, rye, oat, triticale, rice, barley, soybean, peanut, cotton, oil seed rape, including canola and winter oil seed rape, corn, manihot, pepper, sunflower, flax, borage, safflower, linseed, primrose, rapeseed, turnip rape, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato, <SN>Vicia</SN> species, pea, alfalfa, coffee, cacao, tea, <SN>Salix</SN> species, oil palm, coconut, perennial grass, forage crops, or <SN>Arabidopsis thaliana</SN>. The transgenic plant cell, plant, or its part is also derived from a gymnosperm plant, preferably spruce, pine, or fir.
  40. The plant has: (a) an increased biomass production under conditions where water would be limiting for growth for a non-transformed wild type plant cell, plant, or its part; (b) an increased biomass production under conditions of drought and/or desiccation where the conditions would be limiting for growth for a non-transformed wild type plant cell, plant, or its part; or (c) an increased biomass production under conditions of low humidity where the conditions would be limiting for growth for a non-transformed wild type plant cell, plant, or its part.
  41. <USE> USE - The methods are useful for producing a transgenic plant cell, a plant, or its part with increased tolerance and/or resistance to environmental stress and increased biomass production; identifying a compound conferring an increased tolerance and/or resistance to environmental stress and increased biomass production; and producing an agricultural composition. The SRP encoding nucleic acid molecule is useful for preparing a plant cell with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell, plant, or its part. It is also useful as markers for selection of plants or plant cells with an increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant cell. It can also be used as markers for detection of stress in plants or plant cells (all claimed).
  42. <EG> EXAMPLE - No suitable example given.
  43. <XPNO> WO2008142034A2
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