<form name="pyform" method="POST" action="upload.py">
   <input type="file" name="image" /><br />
   <input type="submit" name="submit" value="Submit" />
</form>

#!c:/Python34/python
# -*- coding: UTF-8 -*-
print("Content-Type: text/html")
print()

import cgi,cgitb
cgitb.enable()

form = cgi.FieldStorage()
file = form['image'].value

upload_dir = 'C:/wamp/www/upload/' # esse é o diretório onde vou salvar a imagem

f = open(upload_dir + file, 'w')
f.write(file)
f.close()